About this episode

Mitch Guttman discusses the innovative ChIP-DIP method for high-resolution protein-DNA mapping and its implications in chromatin profiling.

In this episode of the Epigenetics Podcast, we talked with Mitch Guttman from Caltec about ChIP-DIP (ChIP-Done In Parallel). ChIP-DIP is a newly developed approach for high-resolution protein–DNA interaction mapping. The method uses antibody-guided isolation of denaturant-insoluble protein–DNA complexes, resulting in substantially improved specificity and peak definition compared with conventional ChIP-seq. We explore why denaturation resistance is central to the workflow, how the method performs across transcription factors, chromatin regulators, and histone marks, and what experimental parameters determine its success. The conversation also covers current limitations, practical adoption details, and perspectives on how ChIP-DIP fits into the broader landscape of chromatin profiling technologies. References Perez, A. A., Goronzy, I. N., Blanco, M. R., Yeh, B. T., Guo, J. K., Lopes, C. S., Ettlin, O., Burr, A., & Guttman, M. (2024). ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements. Nature genetics, 56(12), 2827–2841. https://doi.org/10.1038/s41588-024-02000-5 Ramani, V. Split-pool barcoding serves up an…

People in this episode

Guest: Mitch Guttman

Topics covered

• protein-DNA interaction
• ChIP-DIP
• chromatin profiling
• transcription factors
• experimental parameters
• gene regulatory elements

Keywords

• ChIP-DIP
• protein-DNA mapping
• chromatin
• transcription factors
• gene regulation
• epigenetics
• high-resolution mapping

Mentioned in this episode

Organizations: Caltec

Books & works: Nature genetics, Nat Genet

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