Listen In - Bitesize Bio Webinar Audios

Are you looking to elevate the performance of your immunoassays? Tune in to this Listen In episode, where experts will share valuable insights to help you optimize your assay selection, production practices, and method analysis. In this podcast, you will learn:• When to choose screening assays versus qualified or verified assays to effectively meet your research objectives• Key techniques that contribute to the reliability and consistency of immunoassays• How to leverage analytical tools to assess immunoassay results for improved experimental outcomes Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-boost-assay Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Learn how to perform a powerful new tissue-clearing method, designed for whole-brain 3D spatial RNA imaging at single cell resolution, and able to provide uniform staining while enhancing tissue transparency in whole organs across multiple species.In this webinar, you will learn:• A groundbreaking tissue-clearing technique for precise whole-brain 3D spatial RNA imaging at single-cell resolution;• How to use it for robust 3D RNA imaging of whole organs of various sizes from different species;• How to uniformly stain whole organs while enhancing tissue transparency and preserving RNA integrity. Watch the full presentation here: https://events.bitesizebio.com/whole-brain-spatial-transcriptomics Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In the life sciences, an astounding amount of unused products and materials end up in landfills instead of contributing to research progress. This issue arises across the product lifecycle, from liquidated biotech start-ups that abandon equipment to over-purchasing and pipeline deprioritization at successful firms. Even well-intentioned suppliers encounter forecasting challenges that leave perfectly good items discarded!In this episode of Listen In, explore the root causes of waste in our industry, as we draw on real-world examples of failed companies, discontinued pipelines, and canceled orders. We will examine the ripple effects this has on both operational efficiency and the broader sustainability goals of life science organizations. We will also introduce Wasteless Bio, a dedicated marketplace designed to keep high-quality, unused products in circulation—helping researchers access discounted reagents and instruments while reducing environmental impact. Whether you are a biotech executive, lab manager, or supplier, you will gain a clearer understanding of how to rescue valuable resources from the trash and reinvest them back into scientific innovation.Watch the full presentation here:Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, we showcase cutting-edge mass spectrometric analysis of mucins, including the characterization of mucinases, enrichment techniques, and complete mucinomic mapping of translationally relevant mucin proteins.Mucins are a diverse family of high molecular weight glycoproteins that play critical roles in protecting and lubricating epithelial surfaces, making them integral to numerous physiological processes and disease states. As a promising class of biomarkers, mucins may offer unique insights into various pathologies, such as cancer and chronic inflammatory diseases. However, their complex structures and extensive glycosylation present significant challenges in their study and application.Mucin-domain glycoproteins are densely O-glycosylated and play key roles in many biological functions. However, their dense O-glycosylation remains enigmatic both in the glycoproteomic landscape and structural dynamics, primarily due to the challenges associated with studying mucin domains. In this episode, you will get an introduction to the (glyco)proteomic techniques used in Mucinomics and learn how proper sample preparation of mucin-containing tissues for mass spectrometry is key to discovery.MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.Watch the full presentation here: Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Immunoassays are pivotal in scientific research, enabling the precise identification and measurement of specific analytes. They pave the way for investigations into disease mechanisms, discovering biomarkers, and evaluating the efficacy of potential therapeutic interventions.The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits.When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design.Additionally, understanding the various assay formats and their data outputs can aid in the immunoassay selection process.In this episode, immunoassay experts give practical guidance in these areas, empowering researchers to achieve more precise and meaningful results, thereby advancing knowledge and understanding in various scientific fields.MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.For Research Use Only. Not For Use In Diagnostic Procedures.Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-strategies-to Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings.Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research.In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation.Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing.In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling.Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously.Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system.Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice.Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy.Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels.Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training.Watch the full presentation here: https://microscopyfocus.com/five-color-sted/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/